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好文推荐:左归丸水煎液对衰老MSCs 的影响
作者:丁富平,张晨,张玉卓,等 来源:转化医学电子杂志 2017-05-08

 

  【摘 要】目的:探讨左归丸水煎液对D⁃半乳糖诱导骨髓间充质干细胞(MSCs)衰老模型的影响. 方法:分离、培养大鼠MSCs,D⁃半乳糖诱导MSCs 建立衰老模型. MTT 法检测衰老MSCs 活性;流式细胞术比较细胞增殖周期的变化;细胞衰老

  β⁃半乳糖苷酶染色和吉姆萨染色比较细胞衰老情况. 结果:模型组MSCs 增殖能力显著低于正常组和左归丸中剂量组,模型组停滞在G1 期细胞数目显著高于正常组和左归丸中剂量组,进入DNA 快速复制期的细胞显著低于正常组和左归丸中

  剂量组,模型组细胞衰老β⁃半乳糖苷酶染色阳性率显著高于正常组和左归丸中剂量组. 结论:左归丸水煎液在一定程度上可促进衰老MSCs 增殖,减少衰老细胞,提示左归丸水煎液对 体外培养诱导的衰老MSCs 有一定的抗衰老作用.

  【关键词】衰老;骨髓间充质干细胞;左归丸;增殖

 

 Effects of Zuogui Pill water decoction on aging bone marrow mesenchymal stem cells

   【Abstract】AIM: To investigate the effect of Zuogui Pill water decoction on bone marrow mesenchymal stem cell (MSCs) aging model induced by D⁃galactose. METHODS: MSCs was isolated and cultured, and ageing cell model was builded by D⁃galactose

induced MSCs. Activity of MSCs was observed by MTT, and change of cell proliferate cycle was compare by flow cytometry. Condition of cell aging was compared by β-galactosidase staining and giemsa staining of senescent cells. RESULTS: The proliferation capacity of MSCs in model group was significantly lower than those of Zuogui Pill groups and normal group; Cell number of MSCs stagnated in the G1 phase in model group was significantly higher than those of Zuogui Pill groups and normal group; Cell number of MSCs entering the period of DNA rapid replication was lower than those of Zuogui Pill groups and normal group; Positive rate of β⁃galactosidase staining aging cells in model group was higher than Zuogui Pill groups and normal group. CONCLUSION: Zuogui Pill water decoction can improve the proliferation ability of ageing rat MSCs and reduce the number of aging MSCs in some degree, which shows Zuogui Pill water has the effect of anti⁃aging.

  【Keywords】aging; MSCs; Zuogui Pill; proliferation

全文阅读:http://www.ejotm.com/ch/reader/create_pdf.aspx?file_no=20161205001&flag=&journal_id=zhyxdzzz&year_id=2017