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OmicsLink™ shRNA Clone Collections
作者: 来源: 2014-03-15

  OmicsLink™ shRNA clone collections include lentiviral and non-viral vector-based shRNA constructs against genome-wide human, mouse and rat genes. shRNA of varying lengths (19 to 29 bases) were designed using a proprietary algorithm to make shRNA expression constructs that have high knockdown efficiency with minimal off-target effect.

  Guaranteed shRNA knockdown

  A set of four expression constructs is offered against every target gene with the guarantee that at least one of the four will have a knockdown effect of 70% or more on corresponding gene expression as determined by qRT-PCR, otherwise the constructs will be replaced one time free of charge. For more about our guaranteed shRNA knockdown, refer to warranty and cancellation policy here.

  All cell types covered

  Lentiviral and non-viral vector option allows transfection or transduction of shRNA into difficult-to-transfect cells as well conventional dividing cell lines.

  Multiple delivery formats

  shRNA are delivered as 4 individual constructs of 5 µg purified plasmid. Lentivirus for shRNA is also available as a part of custom services. Request a quote for custom shRNA lentivirus production service here.

  Markers and reporters

  Vectors with mCherry or eGFP reporter gene for monitoring transfection or transduction efficiencies. Stable cell selection with puromycin marker.

  Fully sequenced expression cassettes

  Expression cassettes of all shRNA clones are fully sequenced including the promoter, sense and antisense target sequences, hairpin, termination and other linker sequences.

  

Lentiviral and non-viral expression vector-based shRNA clones

 

  Figure 1. Lentiviral and non-viral expression vector-based shRNA clones